牛miR-320a的靶基因预测及生物信息学分析

doi:10.11843/j.issn.0366-6964.2015.11.006

Abstract

Abstract:

The prediction and analysis of bta-miR-320a target genes by bioinformatics were performed to explore its effect on the fat deposition in bovine.In this study，bioinformatics tools such as Promoter Scan，TargetScan，DAVID，Cytoscape and databases for example miRBase，Ensemble，NCBI，miRWalk，et al were used to perform the prediction of the transcription factor binding site and the analysis of its target genes，involved Gene ontology and KEGG pathway.As a result，miR-320(a) was very conservative among various species.There were many different transcription factor binding sites in the bta-miR-320a promoter area.Eighty-four target genes were obtained in total which involved in multiple biological processes such as negative regulation of cell differentiation，regulation of cell cycle and negative regulation of growth and participated in p53，cell cycle and MAPK signal pathways.We assumed that bta-miR-320a is regulated by different transcription factors，and it can also regulate the adipocyte differentiation and fat deposition in bovine by post transcriptional inhibiting of its target genes involved in p53，cell cycle and MAPK signal pathways.

CLC Number:

S823
S813.3

GUO Yun-tao，ZHANG Xiu-xiu，MIAO Xiang-yang. Target Gene Prediction and Bioinformatics Analysis of bta-miR-320a[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(11): 1952-1960.

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Target Gene Prediction and Bioinformatics Analysis of bta-miR-320a

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